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More selective interactions of nanoparticles with cells would substantially increase their potential for diagnostic and therapeutic applications.

This multiligand binding leads to five orders of magnitude increased-receptor affinities, compared with free ligand, in displacement studies.

Nanoparticle vaccine delivery platforms are a promising technology for enhancing vaccine immunogenicity.

Protein nanoparticles (PNPs), made entirely from antigen, have been shown to induce protective immune responses against influenza.

The attenuated acidification and pro-inflammatory profile generated by PNPs in DCs demonstrate that physical biomaterial properties can modulate dendritic cell-mediated antigen processing and adjuvancy.

In addition to nanoparticles’ enhancement of DC antigen uptake, our work suggests that vaccine nanoparticle size and coating are uptake-independent modulators of immunogenicity.