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Loading the column involves placing the sample directly onto the top of the column and allowing it to absorb onto the silica.

To load the column: If your compound has poor solubility in the solvent system that gives the best separation, it is possible to dry-load the sample onto the column. If the flow rate is too slow, diffusion processes will lead to band widening (Fig. If it is too fast, there is not enough time for equilibration and the compound will be forced down the column, leaving a long tail behind (Fig. For small-diameter columns, the optimal rate is lower than that for larger-diameter columns.

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